Program blastn blastp blastx tblastn tblastx Database original assemble Scaffolds(Raw Scaffolds) Selection Scaffolds Gap fill (Complite Scaffolds) Gene DNA Gene Protein

Enter sequence below in FASTA format

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Expect 0.0001 0.01 1 10 100 1000    Matrix PAM30 PAM70 BLOSUM80 BLOSUM62 BLOSUM45 Perform ungapped alignment

Query Genetic Codes (blastx only) Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial; ... (4) Invertebrate Mitochondrial (5) Ciliate Nuclear; ... (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15)

Database Genetic Codes (tblast[nx] only) Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial; ... (4) Invertebrate Mitochondrial (5) Ciliate Nuclear; ... (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15)

Frame shift penalty for blastx 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 25 30 50 1000 No OOF

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