Lentinula edodes (shiitake/ Pyogo mushroom), the second most widely cultivated
mushroom, is valued for foods and its pharmacological characteristics. In particular, recent
reports have highlighted its medicinal properties, as well as the critical effects of its
carbohydrates, including lentinan, on the immune.
In addition to being an effective bio-reagent for degrading lignocellulosic wastes, L. edodes has also been shown to possess
biotechnological utility. Genome sequencing and functional annotation will provide valuable
information for establishing key molecular genetic markers that can be used to improve the
quality and usage of this mushroom.
In this project, we report whole genome de novo
sequencing and gene annotation of L. edodes monokaryon B17.
For whole genome sequencing, 61.6 X long reads (on average, 8,011 bp) were produced
using the PacBio RS II system (Pacific Biosciences); 120 X long-mated pair reads (5 and 10 kb
libraries) and 84.6 X short-insert reads (500 bp library) were produced using the Illumina
HiSeq2500 system by 100 bp paired-end sequencing. The resulting assembly consists of 31
scaffolds constituting 46.1 Mb with 0.45% Ns (N50, 3.66 Mb; N90, 0.81 Mb; the longest
scaffold, 5.85 Mb). Finally, 13,028 gene models were predicted by combining the results of
transcriptome and protein sequence alignments and ab initio prediction, giving an average
of 5.78 exons per gene.
